Histone H4 Total Quantification Kit (Colorimetric) (ab156909)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 30 min
- Sample type: Cell culture extracts, Nuclear Extracts, Tissue Extracts
- Sensitivity: 0.5 ng/well
Overview
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Product name
Histone H4 Total Quantification Kit (Colorimetric)
See all Histone H4 kits -
Detection method
Colorimetric -
Sample type
Cell culture extracts, Tissue Extracts, Nuclear Extracts -
Assay type
Quantitative -
Sensitivity
>= 0.5 ng/well -
Range
50 ng - 1000 ng -
Assay time
3h 30m -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
Abcam's Histone H4 Total Quantification Kit (Colorimetric) is a complete set of essential components which enables an experimenter to colorimetrically quantify levels of histone H4 proteins independent of their modified states. It can also be used for normalizing the modified histone H4 content of samples when run in parallel with Abcam's histone modification quantification kit series.
The kit is suitable for specifically measuring total histone H4 using human, mouse, and rat samples, including fresh and frozen tissues and cultured adherent and suspension cells.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 48 tests 96 tests 1000X Capture Antibody 1 x 5µl 1 x 10µl 10X Wash Buffer 1 x 14ml 1 x 28ml 2000X Signal Reporter 1 x 6µl 1 x 12µl 8-Well Assay Strips (with Frame) 1 x 6 units 1 x 12 units Color Developer 1 x 5ml 1 x 10ml Enhancer Solution 1 x 6µl 1 x 12µl Histone Assay Buffer 1 x 4ml 1 x 8ml Standard Control, 100 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 5ml 1 x 10ml -
Research areas
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Relevance
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Cellular localization
Nuclear -
Alternative names
- H4/m
- H4FA
- H4FM
see all -
Database links
- Entrez Gene: 8294 Human
- Entrez Gene: 319155 Mouse
- Entrez Gene: 64627 Rat
- Omim: 602833 Human
- SwissProt: P62805 Human
- SwissProt: P62806 Mouse
- SwissProt: P62804 Rat
Images
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Functional Assay: ab156909 Histone H4 Total Quantification Kit (Colorimetric)
Total H4 per mg of extracted histones from HeLa control cells (C) or cells treated with 10 micromolar sodium butyrate (But) (extracted using ab113476; 50-500 ng per well) (duplicates +/- SD).
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Functional Assay: ab156909 Histone H4 Total Quantification Kit (Colorimetric)Total H4 per mg of extracted histones from murine tissue preparations (extracted using ab113476; 50-500 ng per well) (duplicates +/- SD).
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Functional Assay: ab156909 Histone H4 Total Quantification Kit (Colorimetric)Standard curve with background signal subtracted (duplicates; +/- SD).
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Histone H4 QuantificationHistone nuclear extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the ODs generated from Histone H4 are measured.
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Illustrated Standard CurveFigure 1. Illustrated standard curve generated with H4 standard.