Heparin Affinity Resin - Amintra (ab270307)
Overview
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Product name
Heparin Affinity Resin - Amintra -
General notes
Heparin Affinity Resin - Amintra (ab270307) is an affinity chromatography medium that is used for the purification of biomolecules that show affinity to heparin, such as anti-thrombin III, coagulation factors and other plasma proteins, DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.
This product is manufactured by Expedeon, an Abcam company. Expedeon product code AHP0025 was previously called Amintra Heparin Affinity Resin - 25 ml medium (50 ml in 50% slurry) and is the same as the 25 mL size of this product. Expedeon product code AHP0005 was previously called Amintra Heparin Affinity Resin - 5 ml medium (10 ml in 50% slurry) and is the same as the 5 mL size.
The base matrix is made of cross linked porous agarose. The particles have an open pore structure with good mass transfer properties to large protein molecules. Heparin is immobilized to the base matrix by the reduced amination method.
The medium shows high mechanical rigidity, so it can be operated at high flow velocities with moderate pressure drops. The medium is compatible with most of the chemical reagents commonly used in biological systems.
Characteristics
Matrix Agarose Functional group Heparin of porcine origin Ligand density 4–5 mg / ml Particle size 50–150µm pH stability 4–13 (short term) and 4–12 (long term) Working temperature +4°C to +30°C Chemical stability Compatible with commonly used buffers,
6M guanidine-HCl, 8M ureaStorage 0.05M sodium acetate in 20% denatured
ethanol
Properties
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Form
Liquid -
Storage instructions
Shipped at Room Temperature. Store at +4°C. Do Not Freeze. Please see notes section. -
Storage buffer
Constituents: 20% Ethanol, 0.41% Sodium acetate -
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