Hemin Assay Kit (ab65332)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Overview
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Product name
Hemin Assay Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Assay time
0h 30m -
Product overview
Hemin Assay Kit (ab65332) utilizes peroxidase activity in the presence of hemin to provide a simple, exquisitely sensitive assay which causes the conversion of a colorless probe to a strongly colored (? = 570) compound. Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range.
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Notes
Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. It can be detected in various body fluids such as saliva, urine and csf under various pathological states. Free hemin exists in cells at a very minute concentrations (
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 100 tests Enzyme Mix Blue 1 vial Hemin Assay Buffer WM 1 x 25ml Hemin Probe (in DMSO) Red 1 x 0.2ml Hemin Standard (1 nM) (Lyophilised) Yellow 1 vial Hemin Substrate Green 1 x 1ml -
Research areas
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Relevance
Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. Hemin level has been used as an indicator of possible pathological conditions when found in the urine or faeces. It plays regulatory functions for enzyme activities.
Images
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Functional studies - ab65332 Urasaki Y., PLoS One 9(6), Fig 4D. doi: 10.1371/journal.pone.0099728 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Uraskai et al investigates the effect of perturbations to uridine homeostasis on heme-deficiency response pathway. Mouse liver hemin concentration was measured using ab65332.
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Functional studies - ab65332 Hemin Assay KitHemin measured in various biofluids showing concentration (nM)
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Functional Studies - Hemin Assay Kit (ab65332)A: Time course of development of color and B: Standard Curve at 30 minutes for Hemin standard.