Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human PAPPA ELISA Kit (ab235647)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 8.89 pg/ml
  • Range: 156.25 pg/ml - 14000 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human PAPPA ELISA Kit
    See all PAPP A kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 3 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Serum 8 5.7%

  • Sample type

    Serum, Tissue Extracts, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    8.89 pg/ml

  • Range

    156.25 pg/ml - 14000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 109 103% - 115%
    Tissue Extracts 105 100% - 112%
    Hep Plasma 96 95% - 96%
    EDTA Plasma 95 85% - 113%
    Cit plasma 86 84% - 87%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human PAPPA ELISA Kit (ab235647) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAPPA protein in cit plasma, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAPPA with 8.89 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human PAPPA Capture Antibody 1 x 600µl
    10X Human PAPPA Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human PAPPA Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.

  • Tissue specificity

    High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.

  • Sequence similarities

    Belongs to the peptidase M43B family.
    Contains 5 Sushi (CCP/SCR) domains.

  • Developmental stage

    Present in serum and placenta during pregnancy; levels increase throughout pregnancy.

  • Post-translational
    modifications

    There appear to be no free sulfhydryl groups.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • ASBABP2
    • Aspecific BCL2 ARE binding protein 2
    • Differentially placenta 1 expressed protein
    • DIPLA1
    • EC 3.4.24.79
    • IGF dependent IGFBP 4 protease
    • IGF-dependent IGFBP-4 protease
    • IGFBP 4ase
    • IGFBP-4ase
    • IGFBP4ase
    • Insulin like growth factor dependent IGF binding protein 4 protease
    • Insulin-like growth factor-dependent IGF-binding protein 4 protease
    • PAPA
    • PAPP A
    • PAPP-A
    • PAPP1_HUMAN
    • PAPPA
    • PAPPA1
    • Pappalysin 1 precursor
    • Pappalysin-1
    • Pregnancy Associated Plasma Protein A
    • Pregnancy associated plasma protein A pappalysin 1
    • Pregnancy-associated plasma protein A
    see all

  • Database links

    Images

    • Other - Human PAPPA ELISA Kit (ab235647)
      Other - Human PAPPA ELISA Kit (ab235647)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Human PAPPA Competitor Standard Curve Comparison.
      Human PAPPA Competitor Standard Curve Comparison.

      Standard curve comparison between Human PAPPA SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

    • Example of human PAPPA standard curve in Sample Diluent NS.
      Example of human PAPPA standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of human PAPPA standard curve in 1X Cell Extraction Buffer PTR.
      Example of human PAPPA standard curve in 1X Cell Extraction Buffer PTR.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native PAPPA in human pregnant serum and pregnant serum from a pre-eclampsia donor.
      Interpolated concentrations of native PAPPA in human pregnant serum and pregnant serum from a pre-eclampsia donor.

      The concentrations of PAPPA were measured in duplicates, interpolated from the PAPPA standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant serum 1:500 and pregnant serum pre-eclampsia 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAPPA concentration was determined to be 1669.24 ng/mL in pregnant serum, and 2393.50 ng/mL in pregnant serum pre-eclampsia.

    • Interpolated concentrations of native PAPPA in human placenta extract based on a 250 µg/mL extract load.
      Interpolated concentrations of native PAPPA in human placenta extract based on a 250 µg/mL extract load.

      The concentrations of PAPPA were measured in duplicate and interpolated from the PAPPA standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAPPA concentration was determined to be 2862.86 pg/mL.

    • Sandwich ELISA - Human PAPPA ELISA Kit (ab235647)
      Sandwich ELISA - Human PAPPA ELISA Kit (ab235647)
      To learn more about the advantages of recombinant antibodies see here.

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