Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23588-44] to MASP2 - BSA and Azide free
  • Suitable for: WB, IHC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free
    See all MASP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23588-44] to MASP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHCmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma; human kidney tissue lysate. IHC-P: Human liver, kidney and colon tissue; mouse liver tissue; rat liver tissue.

  • General notes

    ab277528 is the carrier-free version of ab277520. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277528 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)

    This data was developed using ab277520, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling MASP2 with ab277520 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human liver (PMID: 16395704). Counter stained with Hematoxylin. The section was incubated with ab277520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)
    Western blot - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)
    All lanes : Anti-MASP2 antibody [EPR23588-44] (ab277520) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 3 : A549 (Human lung carcinoma epithelial cell ), whole cell lysate
    Lane 4 : Human kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

    Predicted band size: 76 kDa
    Observed band size: 19,50,75 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab277520, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight is consistent with what has been described in the literature (PMID: 16116205, 23785123, 21871896, 15078867).

    Negative control: A549 (PMID:17131120).
    Low expression: HepG2 (PMID:17131120).

    Exposure times: Lanes 1-3, 15 seconds;

    Lane 4: 180 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)

    This data was developed using ab277520, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling MASP2 with ab277520 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human kidney (PMID: 30286468). Counter stained with Hematoxylin. The section was incubated with ab277520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)

    This data was developed using ab277520, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MASP2 with ab277520 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma of human colon (PMID: 32771488). Counter stained with Hematoxylin. The section was incubated with ab277520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)

    This data was developed using ab277520, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling MASP2 with ab277520 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse liver. Counter stained with Hematoxylin. The section was incubated with ab277520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)

    This data was developed using ab277520, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MASP2 with ab277520 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat liver. Counter stained with Hematoxylin. The section was incubated with ab277520 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)
    Anti-MASP2 antibody [EPR23588-44] - BSA and Azide free (ab277528)

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