Mouse Beta-2-Microglobulin ELISA Kit (ab223590)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.165 pg/ml
- Range: 3.125 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Beta-2-Microglobulin ELISA Kit
See all beta 2 Microglobulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.8% Inter-assay Sample n Mean SD CV% Serum 3 3.2% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.165 pg/ml -
Range
3.125 pg/ml - 200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 114 110% - 118% Urine 98 95% - 100% Serum 102 97% - 106% Hep Plasma 93 82% - 103% EDTA Plasma 103 101% - 107% Cit plasma 102 101% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Beta-2-Microglobulin ELISA Kit (ab223590) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Beta-2-Microglobulin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Beta-2-Microglobulin with 1.165 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Beta-2-Microglobulin is the light chain of the class I major histocompatibility complex (MHC); it forms heterodimers with classical (HLA-A, -B, -C) and non-classical (HLA-E, -F, -G) class I MHC heavy chains and with class I non-MHC heavy chains (CD1). Beta-2-Microglobulin is involved in the presentation of peptide antigens to the immune system. Beta-2-Microglobulin can dissociate from the MHC and circulate in complex with alpha 2-Macroglobulin.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Beta-2-Microglobulin Capture Antibody 1 x 600µl 10X Mouse Beta-2-Microglobulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Denaturant 1 x 500µl Mouse Beta-2-Microglobulin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
Sequence similarities
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Post-translational
modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients. -
Cellular localization
Secreted. Detected in serum and urine. - Information by UniProt
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Alternative names
- B2M
- B2MG_HUMAN
- Beta 2 microglobin
see all -
Database links
- Entrez Gene: 12010 Mouse
- SwissProt: P01887 Mouse
- Unigene: 163 Mouse
Images
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Other - Mouse Beta-2-Microglobulin ELISA Kit (ab223590)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Beta-2-Microglobulin standard curve in Sample Diluent NS + EnhancerBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Beta-2-Microglobulin in mouse serum, and plasma samplesThe concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:100,000, plasma (citrate) 1:100,000, plasma (heparin) 1:100,000, and plasma (EDTA) 1:100,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 19.48 µg/mL in neat serum, 15.15 µg/mL in neat plasma (citrate), 18.74 µg/mL in neat plasma (heparin), and 15.87 µg/mL in neat plasma (EDTA).
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Interpolated concentrations of native Beta-2-Microglobulin in mouse urine and lung tissue culture supernatant samplesThe concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:100, lung supernatant 1:5,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 16.12 ng/mL in neat urine, 440.7 ng/mL in neat lung tissue culture supernatant.
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Sandwich ELISA - Mouse Beta-2-Microglobulin ELISA Kit (ab223590)To learn more about the advantages of recombinant antibodies see here.
