Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed (ab98416)
Key features and details
- Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3 ®), pre-adsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt, IHC-Fr
Overview
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Product name
Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed
See all IgG secondary antibodies -
Description
Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3 ®), pre-adsorbed -
Host species
Goat -
Target species
Rat -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cyt, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details -
Conjugation
Cy3 ®. Ex: 552nm, Em: 565nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed (ab98416) Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Rat anti-EndoA alpha 1, 1:100
Rabbit anti-laminin alpha 1, 1:400
Secondary antibody:Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3®) (ab98416), 1:100
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
Nuclei were counterstained with SYTO 16 -
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