Goat Anti-Rat IgG Fc (DyLight® 488) preadsorbed (ab96971)
Key features and details
- Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 488), pre-adsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Overview
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Product name
Goat Anti-Rat IgG Fc (DyLight® 488) preadsorbed
See all IgG secondary antibodies -
Description
Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 488), pre-adsorbed -
Host species
Goat -
Target species
Rat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Reduced cross-reactivity to human and mouse IgG was detected.
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Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Minimal
cross-reactivity
Human, Mouse more details -
Conjugation
DyLight® 488. Ex: 493nm, Em: 518nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Goat Anti-Rat IgG Fc (DyLight® 488) preadsorbed (ab96971)
Overlay histogram showing peripheral blood lymphocytes stained with ab30446 (red line). The cells were incubated with the antibody (ab30446, 1μg/1x10^6 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) at 1/250 dilution for 30 min at 4°C. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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Goat Anti-Rat IgG Fc (DyLight® 488) preadsorbed (ab96971)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.