Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab202372)
Key features and details
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, Flow Cyt, ICC/IF, IHC-Fr, WB
Overview
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Product name
Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, ICC/IF, IHC-Fr, WBmore details -
Minimal
cross-reactivity
Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details -
Conjugation
DyLight® 488. Ex: 493nm, Em: 518nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab202372)
Immunofluorescent analysis of Human stomach carcinoma labeling PCNA with purified rabbit anti-PCNA antibody at 1/100 dilution followed by ab202372 secondary antibody at 1/100 dilution (green). Counterstain: Dapi (Blue). Images represent different lots of ab202372.
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Western blot - Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab202372)All lanes : cocktail of rabbit anti-Actin and mouse anti-hnRNP at 1 µg/ml
Lane 1 : HeLa cell lysate at 30 µg
Lane 2 : HeLa cell lysate at 15 µg
Secondary
All lanes : cocktail of Dylight® 488-conjugated goat anti-rabbit (ab202372) (blue) and Dylight® 680-conjugated goat anti-mouse (red) at 0.5 µg/ml