Goat Anti-Human IgG H&L (FITC) (ab8503)
Key features and details
- Goat Anti-Human IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Suitable for: Flow Cyt
Overview
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Product name
Goat Anti-Human IgG H&L (FITC)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
Antibodies were raised in goat to human IgG, IgM and IgA -
Tested applications
Suitable for: Flow Cytmore details -
Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Specific antibodies were absorpted by incubation with Sepharose-bound human immuno- globulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human immunoglobulins a minimum of 65% protein bound. -
Conjugation notes
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
Clonality
Polyclonal -
General notes
F/P ratio is 4-6 mol Fluorescein per 1 mol of goat Ig. There is low background fluorescence in normal tissue in contrast to cases of auto immune desease where Ig's are depostited. -
Research areas