Anti-Wnt4 antibody [9HCLC] (ab277798)
![Anti-Wnt4 antibody [9HCLC] (ab277798)](https://www.abcam.com/ps/products/277/ab277798/Images/ab277798-4-antiWNT4recombinantpolyclonalantibody9hclcWB.jpg "Anti-Wnt4 antibody [9HCLC] (ab277798)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [9HCLC] to Wnt4
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-Wnt4 antibody [9HCLC]
See all Wnt4 primary antibodies -
Description
Rabbit recombinant multiclonal [9HCLC] to Wnt4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Wnt4 aa 50-150.
Database link: P56705 -
Positive control
- WB: Jurkat, K562, HCT 116, PANC1 and HeLa whole cell extracts; A-431 cells. ICC: HepG2 cells.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
9HCLC -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Wnt4 antibody (ab277798)All lanes : Anti-Wnt4 antibody [9HCLC] (ab277798) at 1 µg/ml
Lane 1 : Jurkat whole cell extracts
Lane 2 : K562 whole cell extracts
Lane 3 : HCT 116 whole cell extracts
Lane 4 : PANC1 whole cell extracts
Lane 5 : HeLa whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate at 1/2500 dilution
Predicted band size: 39 kDa
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Western blot - Anti-Wnt4 antibody (ab277798)All lanes : Anti-Wnt4 antibody [9HCLC] (ab277798) at 1 µg/ml
Lane 1 : A-431 cells untransfected
Lane 2 : A-431 cells transfected with non-specific scrambled siRNA
Lane 3 : A-431 whole cell extract with WNT4 knockdown
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate at 1/4000 dilution
Predicted band size: 39 kDa
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Immunocytochemistry - Anti-Wnt4 antibody (ab277798)Immunofluorescence was performed on fixed and permeabilized HepG2 cells for detection of Wnt4 using Anti-Wnt4 Recombinant Rabbit Multiclonal Antibody (ab277798, 0.5 μg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of Wnt4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Wnt4 protein. Panel e) represents control cells with no primary Antibody to assess background.
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![Anti-Wnt4 antibody [9HCLC] (ab277798)](https://www.abcam.com/ps/products/277/ab277798/Images/ab277798-5-benefits-of-recombinant-antibodies.jpg)
Anti-Wnt4 antibody [9HCLC] (ab277798)
