Glyoxalase II Activity Kit (Colorimetric) (ab273319)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Cell Lysate, Purified protein, Tissue Homogenate, Whole Blood
Overview
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Product name
Glyoxalase II Activity Kit (Colorimetric)
See all GLO2 kits -
Detection method
Colorimetric -
Sample type
Whole Blood, Cell Lysate, Tissue Homogenate, Purified protein -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Glyoxalase II Activity Assay Kit (ab273319) utilizes the ability of an active Glyoxalase II (GloII) to cleave a substrate while producing D-lactate. The produced D-Lactate reacts with the chromophore generating a stable signal that can be easily quantified at 450 nm using a microplate reader.
Our assay kit is simple, sensitive and can detect as low as 0.6 mU/ml of Glo-II activity in biological samples.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests D-Lactate Standard (100 mM) 1 x 100µl Enzyme Mix (Lyophilized) 1 vial GloII Assay Buffer 1 x 25ml GloII Positive Control 1 x 8µl GloII Probe (Lyophilized) 2 vials GloII Substrate (Lyophilized) 1 vial -
Research areas
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Relevance
Hydroxyacylglutathione hydrolase (HAGH) is a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. -
Cellular localization
Cytoplasmic and Mitochondrial -
Alternative names
- GLO2
- GLX II
- Glyoxalase II
see all
Images
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Example dataGloII Activity in rat liver tissue extracts (1.5 µg protein).
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Example dataMeasurement of GloII activity in human whole blood (6 µl, 1: 50 dilution), human red blood cells (4 µl, 1:100 dilution), HepG2 cell lysates (2 µg protein) and rat liver tissue extracts (1.5 µg protein). All assays were performed following kit protocol.