Glucose Oxidase Assay Kit (Fluorometric) (ab138884)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Other biological fluids, Plasma, Urine
- Sensitivity: 0.05 mU/ml
Overview
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Product name
Glucose Oxidase Assay Kit (Fluorometric)
See all Glucose Oxidase kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Urine, Plasma, Other biological fluids -
Assay type
Enzyme activity -
Sensitivity
0.05 mU/ml -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Glucose Oxidase Assay Kit ab138884 uses a quick and sensitive fluorometric assay for the measurement of glucose oxidase activity. It can be performed in a 96-well or 384-well microtiter plate format and is readily adapted to automation without a separation step.
The glucose oxidase assay protocol uses a red dye which enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at 576 nm.
Glucose oxidase assay protocol summary:
- add standards and samples to wells
- add reaction mix to wells and incubate for 10-30 min at 37ºC
- analyze with a microplate reader -
Notes
Glucose oxidase is a dimeric protein that catalyzes the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid. It is widely used for the determination of glucose in body fluids and in removing residual glucose and oxygen from beverages, food and other agricultural products. Furthermore, Glucose oxidase is commonly used in biosensors to detect glucose.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests AbRed Indicator 1 vial Assay Buffer 1 x 50ml DMSO 1 x 200µl Glucose oxidase 1 vial Glucose standard 1 vial Horseradish Peroxidase 1 vial -
Research areas
Images
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Standard Sample Curve for Glucose OxidaseGlucose oxidase dose response was measured with ab138884 in a solid black 96-well plate using a microplate reader. As low as 0.05 mU/ ml glucose oxidase was detected with 30 minutes incubation (n=3).
