Glucagon-Like Peptide 2 ELISA Kit (ab222863)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.281 ng/ml - 18 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat, Human, Pig, Monkey
Overview
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Product name
Glucagon-Like Peptide 2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 1 20 4.4% Plasma 2 20 5.2% Plasma 3 20 5% Inter-assay Sample n Mean SD CV% Plasma 1 20 9.7% Plasma 2 20 10.4% Plasma 3 20 10% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.2 ng/ml -
Range
0.281 ng/ml - 18 ng/ml -
Recovery
97 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat, Human, Pig, Monkey
Does not react with: Rabbit, Cow -
Product overview
The Glucagon-Like Peptide 2 ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab222863) kit is designed for detection of GLP-2 in plasma, serum, and cell culture samples.
The kit has been validated using rat, human, swine, and monkey samples. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures GLP-2 in approximately 4 hours. A polyclonal antibody specific for GLP-2 has been pre-coated onto a 96-well microplate with removable strips. GLP-2 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for GLP-2, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Glucagon-like peptide 2 (GLP-2) is a thirty-three amino acid peptide that is secreted by intestinal endocrine cells alongside GLP-1. GLP-2 and GLP-1 are co-secreted from enteroendocrine L-cells located in distal intestine in response to enteral nutrient ingestion, particularly fats and carbohydrates. GLP-2 secretion is mediated by direct nutrient stimulation of the L-cells and indirect action from enteroendocrine and neural inputs. GLP-2 acts to enhance nutrient absorption by inhibiting gastric motility and secretion and by stimulating nutrient transport.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated GLP-2 Antibody 1 x 120µl Anti-GLP-2 coated Microplate (12 x 8 wells) 1 unit Chromogen Substrate 1 x 7ml GLP-2 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Relevance
Human GLP2 (glucagon like peptide 2) is a peptide hormone of the glucagon family produced by the L cells of the intestial mucosa and originates from the same prohormone as glucagon. GLP2 has been shown to be an important growth factor for the intestinal mucosa. -
Cellular localization
Secreted -
Alternative names
- GCG
- GLP 2
- Glucagon like peptide 2
- GRPP
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Database links
- Entrez Gene: 2641 Human
- Entrez Gene: 24952 Rat
- Omim: 138030 Human
- SwissProt: P01275 Human
- SwissProt: P01274 Pig
- SwissProt: P06883 Rat
- Unigene: 516494 Human
- Unigene: 54383 Rat
Images
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Glucagon-Like Peptide 2 Standard Curve Example
Example of Glucagon-Like Peptide 2 standard curve in 1X Diluent M. Background-subtracted data values (mean +/- SD) are graphed.