GFP Quantification Kit (ab235672)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Cell Lysate, Tissue Extracts, Tissue Homogenate, Tissue Lysate
Overview
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Product name
GFP Quantification Kit
See all GFP kits -
Detection method
Fluorescent -
Sample type
Cell culture extracts, Tissue Extracts, Cell Lysate, Tissue Homogenate, Tissue Lysate -
Assay type
Quantitative -
Range
0.01 µg/ml - 10 µg/ml -
Product overview
The GFP Quantification Kit (ab235672) quantifies GFP in a 96 microplate format for a wide array of cells and tissues.
This kit can detect a wide range of GFP concentrations (0.01-10 μg/ml). Cells or tissues can be homogenized directly in the GFP Assay Buffer, the quantity of GFP is then determined by comparing the fluorescence with that of the GFP standard. Ex/Em = 488/507 nm.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests GFP Assay Buffer 1 x 25ml GFP Quench Solution 1 x 1ml GFP Standard (100 µg) 1 vial -
Research areas
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Relevance
Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm. -
Alternative names
- GFP
- Green fluorescent protein
Images
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Example Data
GFP standard curve.
