Fyn overexpression 293T lysate (whole cell) (ab94223)
Overview
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Product name
Fyn overexpression 293T lysate (whole cell)
See all Fyn lysates -
General notes
ab94223 is a 293T cell transfected lysate in which Human Fyn has been transiently over-expressed using a pCMV-Fyn plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain. Tissue specificity: Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
Images
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Western blot - Fyn overexpression 293T lysate (whole cell) (ab94223)ab94223 at 15µg/lane on an SDS-PAGE gel.
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Western blot - Fyn overexpression 293T lysate (whole cell) (ab94223)All lanes : Anti-Fyn antibody (ab76934) at 1/500 dilution
Lane 1 :Fyn overexpression 293T lysate (whole cell) (ab94223)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
