Fluo-5 AM, Fluorescent labeling reagent (Fluo-4 analogue) (ab241083)
Key features and details
- A Fluo-4 analogue with lower affinity for calcium for detecting intracellular calcium levels. Cell-permeable.
- Purity: > 98%
DMSO
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Fluo-5 AM, Fluorescent labeling reagent (Fluo-4 analogue) -
Description
A Fluo-4 analogue with lower affinity for calcium for detecting intracellular calcium levels. Cell-permeable. -
Alternative names
- Fluo-5F AM
- Fluo5F, AM
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Biological description
Fluo-5 AM is an analogue of Fluo-4 with reduced calcium binding affinity (Kd = 2.3 μM). Suitable for detecting intracellular calcium levels in the range 1 μM to 1 mM. Cells can be loaded with Fluo-5 AM by adding the dissolved indicator directly to cells in culture dishes.
Fluo-5 AM has excitation and emission wavelengths at 494 and 516 nm respectively. Compatible with excitation by argon-ion lasers at 488 nm. Fluorescence inceases by >100-fold upon binding calcium.
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Purity
> 98% -
Chemical structure
Properties
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Excitation
494nm -
Emission
516nm -
Chemical name
4-(6-Acetoxymethoxy-2,7-difluoro-3-oxo-9-xanthenyl)-4′-fluoro-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester
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Molecular weight
1100.91 -
Molecular formula
C50H47F3N2O23 -
PubChem identifier
24802868 -
Storage instructions
Shipped at Room Temperature. Store at -20°C. Store In the Dark. Store under desiccating conditions. -
Solubility overview
DMSO
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c5cccc(F)c5OCCOc1cc(ccc1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=2c4cc(F)c(cc4OC3=CC(=O)C(F)=CC=23)OCOC(C)=O -
Source
Synthetic
