FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)
![FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)](https://www.abcam.com/ps/products/237/ab237453/Images/ab237453-398935-AntiCD3-zeta-phospho-Y83-antibody-EP7762Y-FITC-immunocytochemistry-jurkat-human.jpg "FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- FITC Rabbit monoclonal [EP776(2)Y] to CD3 zeta (phospho Y83)
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
Overview
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Product name
FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]
See all CD3 zeta primary antibodies -
Description
FITC Rabbit monoclonal [EP776(2)Y] to CD3 zeta (phospho Y83) -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
This antibody detects CD3 zeta phosphorylated on tyrosine 83. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: Pervanadate treated Jurkat cells. Flow Cyt: Pervanadate treated Jurkat cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EP776(2)Y -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)
ab237453 staining CD3 zeta (phospho Y83) in Jurkat cells +/- pervanadate ( 1mM, 30min). The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, then incubated ab237453 at 1/50 dilution (shown in green). Counterstained with ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
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![Flow Cytometry - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)](https://www.abcam.com/ps/products/237/ab237453/Images/ab237453-398934-AntiCD3-zeta-phospho-Y83-antibody-EP7762Y-FITC-flow-cytometry-jurkat-human.jpg)
Flow Cytometry - FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)Flow cytometry overlay histogram showing Jurkat + pervanadate ( 1mM, 30min)(Red) / Untreated control (Green) stained with ab237453. The cells were fixed with 4 % formaldehyde (10 min). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab237453).
Isotype control antibody (Black) was Rabbit IgG, monoclonal [EPR25A] - Isotype Control (FITC) (ab223339) used at the same concentration and conditions as the primary antibody.
Unlabelled sample without incubation with primary antibody and secondary antibody (Blue) was also used as a control.
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![FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)](https://www.abcam.com/ps/products/237/ab237453/Images/ab237453-3-benefits-of-recombinant-antibodies.png)
FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (ab237453)
