FITC Anti-beta Actin antibody [AC-15] (ab6277)
![FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.com/ps/products/6/ab6277/Images/ab6277-12195-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg "FITC Anti-beta Actin antibody [AC-15] (ab6277)")
Key features and details
- FITC Mouse monoclonal [AC-15] to beta Actin
- Suitable for: ICC/IF
- Reacts with: Rat, Chicken
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-beta Actin antibody [AC-15]
See all beta Actin primary antibodies -
Description
FITC Mouse monoclonal [AC-15] to beta Actin -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Rat, Chicken -
Immunogen
Synthetic peptide:
DDDIAALVIDNGSGL
conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). -
Epitope
The N-terminal of the beta isoform of actin -
Positive control
- Cultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd.
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General notes
Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Purified Immunoglobulin -
Primary antibody notes
Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells -
Clonality
Monoclonal -
Clone number
AC-15 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)
ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x20
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![Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.com/ps/products/6/ab6277/Images/ab6277-274544-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)Immunocytochemistry/ Immunofluorescence analysis of chicken fibroblast cells labeling beta Actin with ab6277 at 1/300 dilution. The cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1/300 Anti-beta Actin antibody [AC-15] (FITC) (ab6277).
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![Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)](https://www.abcam.com/ps/products/6/ab6277/Images/ab6277-12194-anti-beta-actin-antibody-ac-15-fitc-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x40 no deconvolution.
