Etanercept ELISA Kit (Enbrel®) (ab237643)
Key features and details
- Sensitivity: 0.1 µg/ml
- Range: 0.1 µg/ml - 3 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
Etanercept ELISA Kit (Enbrel®)
See all Etanercept kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
0.1 µg/ml -
Range
0.1 µg/ml - 3 µg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Etanercept ELISA Kit (Enbrel®) (ab237643) is a highly specific and sensitive kit designed for the in vitro determination of Etanercept in biological matrices such as human serum and plasma. The density of color is proportional to the amount of Etanercept captured from the samples and can be quantified when compared with standard curve.
Etanercept (Enbrel®) is a therapeutic fusion protein specific for Tumor Necrosis Factor-Alpha (TNF-α) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Etanercept specifically binds to TNF alpha and blocks its interaction with cell surface TNF receptors and reduces the inflammation and subsequently improves the patient’s health. Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Assay Buffer 1 x 50ml Etanercept Standard S1 1 x 300µl Etanercept Standard S2 1 x 300µl Etanercept Standard S3 1 x 300µl Etanercept Standard S4 1 x 300µl Etanercept Standard S5 1 x 300µl Etanercept Standard S6 1 x 300µl Etanercept Standard S7 1 x 300µl HRP-conjugate Probe 1 x 12ml Micro ELISA Plate 1 unit Plate sealers 2 units Stop Solution 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml -
Relevance
Etanercept (Enbrel®) is a therapeutic fusion protein specific for Tumor Necrosis Factor-Alpha (TNF-a) and is used to treat rheumatic arthritis, intestinal disorders, dermatological diseases and cancer. Etanercept specifically binds to TNF alpha and blocks its interaction with cell surface TNF receptors and reduces the inflammation and subsequently improves the patient’s health. Drug level quantification can be important to adapt patient prescription or to switch to an alternative TNF inhibitor drug.
Images
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Example of Etanercept standard curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.