Anti-GP210 antibody (ab15601)
Key features and details
- Rabbit polyclonal to GP210
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GP210 antibody -
Description
Rabbit polyclonal to GP210 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Rat
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Immunogen
Fusion protein (Rat) (C terminal).
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Positive control
- rat liver
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General notes
Storage in frost-free freezers is not recommended.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GP210 antibody (ab15601)ab15601 used in Western blot at a 1/1000 with rat liver nuclear envelopes.
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Immunocytochemistry/ Immunofluorescence - Anti-GP210 antibody (ab15601)HeLa cells grown on coverslips were washed twice with PBS and permeabilized with 0.2% Triton X-100 in PBS for 2 min at room temperature. Following 2 PBS washes, the coverslips were fixed with 3.7% formaldehyde and subsequently blocked in 2% skim milk powder in PBS-T (PBS containing 0.1% Tween 20) for 2 hrs. For detection of gp210, ab15601 was diluted 1:500-1:1000 in 2% skim milk powder in PBS-T and incubated on the coverslips for 1 hour at RT. Following incubation with primary antibodies, coverslips were washed three times with PBS-T over a period of 45 min. Antibody binding was detected with donkey anti-rabbit antibodies conjugated to Cy3 at a dilution of 1:2500.