Astana Biomed Group, an authorized Abcam distributor in Central Asia

Endothelin 1 ELISA Kit (ab133030)

Key features and details

  • Sensitivity: 0.41 pg/ml
  • Range: 0.78 pg/ml - 100 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Pig

Overview

  • Product name

    Endothelin 1 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Buffer 20 35.9pg/ml 6.7%
    Buffer 20 2.3pg/ml 8.9%
    Buffer 20 1.1pg/ml 8.8%
    Inter-assay
    Sample n Mean SD CV%
    Buffer 20 35.1pg/ml 8.3%
    Buffer 20 2.5pg/ml 5.9%
    Buffer 20 1.2pg/ml 15.3%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.41 pg/ml

  • Range

    0.78 pg/ml - 100 pg/ml

  • Assay time

    2h 00m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Pig

  • Product overview

    Endothelin 1 ELISA kit (ET-1) is designed for the accurate quantitative measurement of Endothelin 1 in serum, plasma and culture fluids. ET-1 has identical amino acid sequence for Human, mouse, rat, cow, dog, pig, and rabbit.


    Endothelin 1 specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated I Endothelin 1 detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the Endothelin 1 amount of sample captured in plate.

  • Notes

    Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.

    Cross Reactivity

    Compound Cross Reactivity
    Endothelin 1 (ET-1) 100
    ET-2 21
    ET-3 3.6
    Human Big ET-1
    Rat Big ET-1
    Human Big ET-2
    Human Big ET-3 Amide

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

Images

  • Sample resluts
    Sample resluts

    Endothelin 1 measured in biological fluids of helathy species showing quantity (pg) per mL of tested sample. Results obtained from samples that were not extracted. Samples diluted 1-9 fold.

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab133030.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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