Abcam's DoubleStain IHC Kit (ab183278) is designed to use with user supplied mouse and rat primary antibody to detect two distinct antigens on mouse tissue or cell samples. This kit has been tested on paraffin embedded tissue, however it can also be used on frozen specimens or freshly prepared monolayer cell smears. This kit is designed not to give background with most mouse strains however there may be some, especially when using frozen tissue, that require additional blocking.

Double staining is one of most common methods used in immunohistostaining allowing the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit (ab183278) supplies the user with two polymer enzyme conjugates: Mouse HRP Polymer and Rat AP Polymer and two distinct substrates/chromogens, AEC (Red color, used with the Mouse HRP Polymer) and BCIP/NBT (Purple color, used with the Rat AP Polymer). A Primer step is used to increase specificity of antibody staining. Both enzyme conjugates are applied to the specimen at the same time and mixed on the slide. This kit offers simplified steps making for a quicker and easier protocol than those used in a sequential procedure. Abcam's DoubleStain IHC Kit (ab183278) is a non-biotin system that avoids endogenous biotin non-specific binding.

• Kits/ Lysates/ Other
• Kits
• IHC tools
• Multicolor chromogenic