All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : JAR lysate
Lane 4 : Human milk lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 48 kDa

Ab108323 staining ADFP in HepG2 (human hepatocellular carcinoma epithelial cell) cells by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% TritonX-100. Samples were incubated with primary antibody at 1/500 dilution (2.5µg/ml). An Alexa Fluor^® 488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 1/1000 dilution (2µg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594), ab195889 was used as a counterstain antibody at 1/200 dilution (2.5µg/ml). DAPI was used as the nuclear counterstain. Confocal image showing cytoplasmic staining in HepG2 cells. 

Overlay histogram showing Jurkat cells stained with ab108323 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108323, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-ADFP antibody [EPR3713] (ab108323) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 48 kDa