Cytosolic Phospholipase A2 Assay Kit (ab133090)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture extracts, Tissue Extracts
Overview
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Product name
Cytosolic Phospholipase A2 Assay Kit
See all Cytosolic Phospholipase A2 kits -
Detection method
Colorimetric -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Enzyme activity (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Cytosolic Phospholipase A2 Assay Kit (ab133090) can be used to determine the activity of cytosolic Phospholipase A2 (cPLA2) in purified preparations, cell cultures, or tissue homogenates. Use of this assay with preparations containing more than one type of PLA2 will result in the measurement of total PLA2 activity rather than cPLA2 alone.
If you want to detect secretory PLA2, we recommend using Secretory Phospholipase Assay Kit (ab133089).
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 96 tests 96-Well Plate (Colorimetric Assay) 1 unit 96-Well Plate Cover 1 unit Arachidonoyl Thio-PC (Substrate) 2 vials Bee venom PLA2 Control 1 vial Bromoenol Lactone Solution 1 vial cPLA2 Assay Buffer 1 vial DTNB/EGTA 4 vials -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
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Function
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. -
Tissue specificity
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PLA2c domain. -
Domain
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+). -
Post-translational
modificationsActivated by phosphorylation at both Ser-505 and Ser-727. -
Cellular localization
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion. - Information by UniProt
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Alternative names
- Calcium dependent phospholipid binding protein
- CPLA 2
- cPLA2
see all
