CyGEL™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling.
CyGEL™ has many applications in imaging and cell-based screening.
Key features of CyGEL™ include:

1. Convenient immobilization of non-adherent cells.
2. Controllable and rapid transition from liquid to gel, which allows quick preparation of live cells for gel mounting.
3. Optically clear with low autofluorescence.
4. Compatible with GFP and other fluorescent probes such as DRAQ5™ (ab108410).

CyGEL™ is recommended for mounting live de-adherent/ non-adherent cells for microscopy, or mounting live C. elegans and parasites (e.g. leishmania, trypanosomes), which can be recovered afterwards. CyGEL™ is provided as concentrated non-buffered form. Add the concentrated PBS supplement (provided) to prime CyGEL™ prior use.
CyGEL™ is supplied as 8 vials, each containing 500µl, and 1 vial of 40xPBS.
For imaging experiments longer than 2 hours, we recommend using CyGEL Sustain™ (ab109205).