COVID-19 N-Protein Human IgG ELISA Kit (ab274339)
Key features and details
- Range: 1.37 U/ml - 1000 U/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Quantitative
Overview
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Product name
COVID-19 N-Protein Human IgG ELISA Kit
See all COVID-19 N-Protein kits -
Detection method
Colorimetric -
Sample type
Serum -
Assay type
Quantitative -
Range
1.37 U/ml - 1000 U/ml -
Assay duration
Multiple steps standard assay -
Product overview
COVID-19 N-Protein Human IgG ELISA Kit (ab274339) is an in vitro indirect ELISA for the quantitative measurement of human IgG antibody against SARS-CoV-2 N protein in human serum.
Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARS-CoV-2 N protein, which combines with the corresponding antibody present in a sample and Positive Control which used as calibration curve for interpretation purposes. The wells are washed, and biotinylated anti-human IgG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID19 N protein human IgG antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Controls are from an inactivated serum sample which contains SARS-COV-2 N protein human IgG antibody. We do not know the exact amount of SARS-COV-2 N protein human IgG antibody in the Positive Control sample. The Positive Control can be used as calibration curve for interpretation purposes in different assays.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Sample Diluent 1 x 25ml Biotinylated anti-Human IgG Antibody 1 vial HRP-Streptavidin Concentrate 1 vial Positive Control 1 vial SARS-CoV-2 N protein coated Microplate 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
Images
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Example data
Example Positive Control calibration curve.
