Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)
![Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)](https://www.abcam.com/ps/products/270/ab270454/Images/ab270454-381958-AntiPAEP-Glycodelin-antibody-EPR2325187-flow-562-cells.jpg "Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23251-87] to PAEP/Glycodelin
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-PAEP/Glycodelin antibody [EPR23251-87]
See all PAEP/Glycodelin primary antibodies -
Description
Rabbit monoclonal [EPR23251-87] to PAEP/Glycodelin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562 cell lysate and human placenta lysate. IHC-P: Human endometrium tissue. Flow: K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23251-87 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K-562 (Human chronic myelogenous leukemia lymphoblast) cells treated with 2ng/ml TPA for 32h, then together with 300ng/ml BFA for another 16h (Red) / Untreated control (Green) labeling PAEP/Glycodelin with ab270454 at 1/500 dilution (0.1μg) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. The expression level of PAEP/Glycodelin is induced in TPA treated K-562 cell (PMID:8025274).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)](https://www.abcam.com/ps/products/270/ab270454/Images/ab270454-381957-AntiPAEP-Glycodelin-antibody-EPR2325187-ihc-human-endometrium-tissue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling PAEP/Glycodelin with ab270454 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in glandular epithelial cells of human endometrium is observed. The section was incubated with ab270454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)](https://www.abcam.com/ps/products/270/ab270454/Images/ab270454-381956-AntiPAEP-Glycodelin-antibody-EPR2325187-wb-human-placenta.jpg)
Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454) at 1/1000 dilution + Human placenta tissue lysate at 20 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 21 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure Time: 26 secs.
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![Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)](https://www.abcam.com/ps/products/270/ab270454/Images/ab270454-381955-AntiPAEP-Glycodelin-antibody-EPR2325187-wb-human-k562-cell-lysate.jpg)
Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)All lanes : Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454) at 1/1000 dilution
Lane 1 : Untreated K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 µg
Lane 2 : K-562 treated with 2 ng/ml PMA(TPA) for 2 days whole cell lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
The expression level of PAEP is induced in TPA treated K-562 cell (PMID:8025274).
Exposure Time: 3 mins.
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![Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)](https://www.abcam.com/ps/products/270/ab270454/Images/ab270454-5-benefits-of-recombinant-antibodies.png)
Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)