Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Wednesday March 10, 2021

Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23251-87] to PAEP/Glycodelin
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-PAEP/Glycodelin antibody [EPR23251-87]
See all PAEP/Glycodelin primary antibodies
Description

Rabbit monoclonal [EPR23251-87] to PAEP/Glycodelin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562 cell lysate and human placenta lysate. IHC-P: Human endometrium tissue. Flow: K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23251-87
Isotype

IgG
Research areas

Flow Cytometry - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K-562 (Human chronic myelogenous leukemia lymphoblast) cells treated with 2ng/ml TPA for 32h, then together with 300ng/ml BFA for another 16h (Red) / Untreated control (Green) labeling PAEP/Glycodelin with ab270454 at 1/500 dilution (0.1μg) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. The expression level of PAEP/Glycodelin is induced in TPA treated K-562 cell (PMID:8025274).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling PAEP/Glycodelin with ab270454 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in glandular epithelial cells of human endometrium is observed. The section was incubated with ab270454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)

Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454) at 1/1000 dilution + Human placenta tissue lysate at 20 µg

Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 21 kDa

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Exposure Time: 26 secs.
Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)

All lanes : Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454) at 1/1000 dilution

Lane 1 : Untreated K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 µg
Lane 2 : K-562 treated with 2 ng/ml PMA(TPA) for 2 days whole cell lysate 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 21 kDa

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

The expression level of PAEP is induced in TPA treated K-562 cell (PMID:8025274).

Exposure Time: 3 mins.
Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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