Coumarin 343 X azide, Blue fluorophore (ab241084)
Key features and details
- Blue fluorophore capable of forming a FRET pair with fluorescien. Suitable for Click chemistry.
- Purity: > 95%
DMF, DMSO
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Coumarin 343 X azide, Blue fluorophore -
Description
Blue fluorophore capable of forming a FRET pair with fluorescien. Suitable for Click chemistry. -
Biological description
Coumarin 343 X azide is a blue emitting fluorophore with an emission maximum around 480 nm. This dye forms a FRET pair with fluorescein, and can harvest blue light energy for the subsequent transfer to other fluorophores.
The azide derivative can be conjugated with alkynes in a copper-catalyzed and copper-free Click chemistry reactions. The molecule contains a long aminohexanoyl linker that provides separation between the dye, and the azide function.
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Purity
> 95% -
Chemical structure
Properties
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Excitation
437nm -
Emission
477nm -
Chemical name
5-{[6-(3-Azidopropylamino)-6-oxohexylamino]carbonyl}-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraen-4-one
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Molecular weight
480.56 -
Molecular formula
C25H32N6O4 -
Storage instructions
Shipped at Room Temperature. Store at -20°C. Store In the Dark. Store under desiccating conditions. -
Solubility overview
DMF, DMSO
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[N-]=[N+]=N\CCCNC(=O)CCCCCNC(=O)C1=Cc2cc3CCCN4CCCc(c2OC1=O)c34 -
Source
Synthetic
