Comet Assay Kit (3-well slides) (ab238544)
Key features and details
- Detection method: Fluorescent
Overview
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Product name
Comet Assay Kit (3-well slides)
See all DNA damage kits -
Detection method
Fluorescent -
Product overview
Comet Assay Kit (3-well slides) (ab238544) is a fast and sensitive kit for the measurement of cellular DNA damage.
The Comet Assay is a single cell gel electrophoresis assay (SCGE) for simple evaluation of cellular DNA damage. It is a convenient way to screen for general DNA damage, regardless of the source or nature of the damage. Kits include Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Please refer to protocols. -
Components 15 tests 75 tests 10X Lysis Solution 1 x 20ml 1 x 100ml 3-Well Comet Slides 1 x 5 slides 1 x 25 slides Comet Agarose 1 x 15ml 1 x 15ml EDTA Solution 1 x 50ml 1 x 250ml Vista Green DNA Dye, 10000X 1 x 5µl 1 x 5µl -
Research areas
Images
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Comet Assay PrincipleComet Assay Principle
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Epifluorescence Microscopy Visualization of DNA Damage.For Data Analysis please see Section 9 in the protocol booklet.
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Etoposide Treatment of Jurkat Cells.Jurkat cells were untreated (left) or treated (right) with 20 µM Etoposide for 4 hrs before performing Comet Assay (alkaline electrophoresis conditions, 33 V/300 mA for 15 mins).
