Coenzyme A Assay Kit (Fluorometric - Green) (ab138889)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture extracts, Cell Lysate, Tissue Extracts
- Sensitivity: 40 nM
Overview
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Product name
Coenzyme A Assay Kit (Fluorometric - Green)
See all Coenzyme A kits -
Detection method
Fluorescent -
Sample type
Cell culture extracts, Tissue Extracts, Cell Lysate -
Assay type
Quantitative -
Sensitivity
= 40 nM -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Coenzyme A Assay Kit (Fluorometric - Green) (ab138889) provides an ultrasensitive fluorometric assay to quantitate CoA content by detection of the –SH group in CoA.
The fluorogenic CoA green indicator dye used in the kit becomes strongly fluorescent upon reacting with –SH.
The assay kit can detect as little as 4 pmol CoA/ 100 µL assay volume (40 nM). It can be performed in a convenient 96-well or 384-well microtiter-plate format at Ex/Em = 490/520 nm, and easily adapted to automation without a separation step.
Coenzyme A assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate for 10-60 min whilst measuring fluorescence with a microplate readerThe kit is for solution-based inhibitors or activators of Coenzyme A only. Any samples that contain proteins or thiol-containing compounds are not compatible with the kit.
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Notes
Previously called Coenzyme A Detection kit (Fluorometric - Green).
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests CoA Green Indicator 1 vial Assay Buffer 1 x 25ml Coenzyme A Standard 1 x 154µg DMSO 1 x 200µl -
Research areas
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Relevance
Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of Coenzyme A is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-Coenzyme A. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. -
Alternative names
- CoA
- CoASH
- HSCoA
Images
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Sample Standard CurveCoA dose response was measured in a 96-well black plate with Coenzyme A Detection Kit using a microplate reader. As low as 40 nM (4 pmol/well) of CoA was detected with 30 minutes incubation time (n=3).
