Chymotrypsin Assay Kit (Fluorometric) (ab234051)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
Overview
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Product name
Chymotrypsin Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Cell Lysate, Tissue Lysate -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Chymotrypsin Assay Kit (Fluorometric) (ab234051) uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore. The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin.
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Notes
Chymotrypsin (EC 3.4.21.1) is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine).
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Chymotrypsin Assay Buffer 1 x 25ml Chymotrypsin Substrate 1 x 200µl Chymotrypsin Activator 1 vial Chymotrypsin Inhibitor 1 x 80µl Coumarin Standard (1 mM) 1 x 100µl Chymotrypsin Positive Control 1 vial -
Research areas
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Function
Has chymotrypsin-type protease activity and hypocalcemic activity. -
Tissue specificity
Pancreas. -
Involvement in disease
Variations in CTRC influence susceptibility to chronic pancreatitis [MIM:167800]. Chronic pancreatitis is a persistent inflammatory disorder characterized by permanent destruction of the pancreatic parenchyma. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Alternative names
- Caldecrin
- Chymotrypsin C
- Chymotrypsin-C
see all
Images
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Coumarin standard curves showing a range of 0-10 nmol/well.
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Reaction kinetics for positive control and rat pancreatic lysate (9 µg). -
Reaction kinetics for rat spleen lysate (160 µg). -
Coumarin standard curves showing a range of 0-1 nmol/well. -
Chymotrypsin activity with and without inhibitor in rat pancreas and rat spleen.The presence of non-specific chymotrypsin-like proteases in spleen leads to some activity in presence of the selective chymotrypsin inhibitor.
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Reaction kinetics using substrate in the presence of Chymotrypsin or Trypsin.The substrate is cleaved by chymotrypsin but not trypsin, making the assay free from trypsin interference.
