Human Hepsin ELISA Kit (ab267604)
Key features and details
- Sensitivity: 61.5 pg/ml
- Range: 61.44 pg/ml - 15000 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Hepsin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
61.5 pg/ml -
Range
61.44 pg/ml - 15000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 122 108% - 134% Plasma 131 114% - 147% Cell culture media 137 124% - 146% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Hepsin ELISA Kit is designed for the quantitative determination of Hepsin in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Hepsin coated on a 96-well plate. Standards and samples are pipetted into the wells and Hepsin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Hepsin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Hepsin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human Hepsin coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human Hepsin Detection Antibody 2 vials Human Hepsin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Plays an essential role in cell growth and maintenance of cell morphology. -
Tissue specificity
Present in most tissues, with the highest level in liver. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- EC 3.4.21.
- HEPS
- HEPS_HUMAN
see all -
Database links
- Entrez Gene: 3249 Human
- Omim: 142440 Human
- SwissProt: P05981 Human
- Unigene: 182385 Human
Images
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Standard curve
Human Hepsin ELISA kit (ab267604) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
