Chromatin Extraction Kit (ab117152)
Key features and details
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Chromatin Extraction Kit
See all Chromatin Extraction kits -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay time
1h 00m -
Product overview
Chromatin Extraction Kit (ab117152) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests 1000X Protease Inhibitor Cocktail 1 x 110µl 10X Lysis Buffer 1 x 11ml Chromatin Buffer 1 x 11ml Extraction Buffer 1 x 11ml -
Research areas
Images
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Functional Studies - Chromatin Extraction kit (ab117152)ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using ab117152.
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Chromatin Extraction Kit (ab117152)Chromatin was extracted from 3x106 mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A. Chromatin yield was 4 µg chromatin/106 cells. Lane 1: 1kb DNA ladder Lane 2: 300 - 650bp DNA fragments
Image courtesy of anonymous Abreview
