Recombinant human ErbB2 / HER2 (mutated V777L) protein (ab207945)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human ErbB2 / HER2 (mutated V777L) protein
See all ErbB2 / HER2 proteins and peptides -
Biological activity
The specific activity of ab207945 was determined to be 14 nmol/min/mg.
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Purity
> 90 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
KRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKV LGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMA GLGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNW CMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDET EYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDG IPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSE FSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLV PQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPL APSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETD GYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSP GKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQ DPPERGAPPSTFKGTPTAENPEYLGLDVPV -
Predicted molecular weight
110 kDa including tags -
Amino acids
676 to 1255 -
Modifications
mutated V777L -
Tags
GST tag N-Terminus -
Additional sequence information
Cytoplasmic domain (NM_004448).
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.002% PMSF, 0.004% DTT, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human ErbB2 / HER2 (mutated V777L) protein (ab207945)SDS PAGE analysis of ab207945
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Functional Studies - Recombinant human ErbB2 / HER2 (mutated V777L) protein (ab207945)The specific activity of ErbB2 / HER2 (ab207945) was determined to be 33 nmol /min/mg as per activity assay protocol.
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SDS-PAGE - Recombinant human ErbB2 / HER2 (mutated V777L) protein (ab207945)SDS-PAGE analysis of ab207945.
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Functional Studies - Recombinant human ErbB2 / HER2 (mutated V777L) protein (ab207945)Sample Kinase Activity assay plot using ab207945, showing the specific activity to be 14 nmol/min/mg.
