CHRAC-17 overexpression 293T lysate (whole cell) (ab94038)
Overview
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Product name
CHRAC-17 overexpression 293T lysate (whole cell)
See all CHRAC-17 lysates -
General notes
ab94038 is a 293T cell transfected lysate in which human CHRAC-17 has been transiently over-expressed using a pCMV-CHRAC-17 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes.
Protein previously known as DNA Polymerase epsilon.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. Tissue specificity: Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Images
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SDS-PAGE - CHRAC-17 overexpression 293T lysate (whole cell) (ab94038)
ab94038 at 15 µg/lane on an SDS-PAGE gel.
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Western blot - CHRAC-17 overexpression 293T lysate (whole cell) (ab94038)All lanes : Anti-CHRAC-17 antibody (ab88521) at 1/500 dilution
Lane 1 : DNA Polymerase epsilon 293T Transfected Lysate - Positive Control (ab94038)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
