Chicken IL-6 ELISA Kit (ab273258)
Key features and details
- Sensitivity: 0.085 ng/ml
- Range: 0.082 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Chicken
Overview
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Product name
Chicken IL-6 ELISA Kit
See all IL-6 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.085 ng/ml -
Range
0.082 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 96.57 71% - 114% Plasma 76.16 72% - 80% Cell culture media 129.7 116% - 140% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Chicken -
Product overview
Chicken IL-6 ELISA Kit (ab273258) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IL-6 in serum, EDTA plasma (Citrate and heparin have not been validated yet) and cell culture supernatants.
This assay employs an antibody specific for Chicken IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Chicken IL-6 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Chicken IL-6 Antibody 2 vials Chicken IL-6 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all
Images
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Chicken IL-6 ELISA Kit standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Chicken IL-6 ELISA kit (ab273258) Standard curve.
