Anti-ANGPTL2/ARP2 antibody (ab35574)
Key features and details
- Rabbit polyclonal to ANGPTL2/ARP2
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-ANGPTL2/ARP2 antibody
See all ANGPTL2/ARP2 primary antibodies -
Description
Rabbit polyclonal to ANGPTL2/ARP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
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Positive control
- This antibody gave a positive signal in the following lysate: Tagged ANGPTL2/ARP2 recombinant protein.
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General notes
This product was previously labelled as ANGPTL2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab35574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Recombinant fragmentAll applications MouseRatHumanApplication Abreviews Notes WB (2) Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 79 (size of the recombinant protein + tag) kDa (predicted molecular weight: 57 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Induces sprouting in endothelial cells through an autocrine and paracrine action. -
Tissue specificity
Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 23452 Human
- Entrez Gene: 26360 Mouse
- Entrez Gene: 171100 Rat
- Omim: 605001 Human
- SwissProt: Q9UKU9 Human
- SwissProt: Q9R045 Mouse
- Unigene: 653262 Human
- Unigene: 208919 Mouse
see all -
Alternative names
- AI593246 antibody
- Angiopoietin like 2 antibody
- Angiopoietin related protein 2 antibody
see all
Images
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Western blot - Anti-ANGPTL2/ARP2 antibody (ab35574)Anti-ANGPTL2/ARP2 antibody (ab35574) at 1 µg/ml + ANGPTL2/ARP2 Recombinant Protein at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?ab35574 was tested on a recombinant tagged ANGPTL2/ARP2 protein with a predicted molecular weight of 79.6 kDa.
Datasheets and documents
References (2)
ab35574 has been referenced in 2 publications.
- Hirasawa M et al. Angiopoietin-like Protein 2 Is a Multistep Regulator of Inflammatory Neovascularization in a Murine Model of Age-related Macular Degeneration. J Biol Chem 291:7373-85 (2016). IHC . PubMed: 26839315
- He XX et al. Bioinformatics analysis identifies miR-221 as a core regulator in hepatocellular carcinoma and its silencing suppresses tumor properties. Oncol Rep 32:1200-10 (2014). PubMed: 24993451
Images
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Western blot - Anti-ANGPTL2/ARP2 antibody (ab35574)Anti-ANGPTL2/ARP2 antibody (ab35574) at 1 µg/ml + ANGPTL2/ARP2 Recombinant Protein at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?ab35574 was tested on a recombinant tagged ANGPTL2/ARP2 protein with a predicted molecular weight of 79.6 kDa.
