Chicken IL-21 ELISA Kit (ab277406)
Key features and details
- Sensitivity: 4.5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Chicken
Overview
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Product name
Chicken IL-21 ELISA Kit
See all IL-21 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Quantitative -
Sensitivity
4.5 pg/ml -
Range
4.1 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 106.1 82% - 131% Plasma 102.3 77% - 129% Cell culture media 102.7 83% - 124% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Chicken -
Product overview
Chicken IL-21 ELISA kit (ab277406) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IL-21 in serum (Chicken IL-21 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma EDTA (Citrate and Heparin have not been validated yet) and cell culture supernatants.
This assay employs an antibody specific for Chicken IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and Chicken IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Chicken IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Chicken IL-21 Antibody 2 vials Anti-Chicken IL-21 coated Microplate 1 unit Chicken IL-21 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. -
Tissue specificity
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CVID11
- IL 21
- IL-21
see all
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.