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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin

Anti-NUAK2 antibody (ab224079)

Anti-NUAK2 antibody (ab224079)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to NUAK2
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-NUAK2 antibody
    See all NUAK2 primary antibodies
  • Description

    Rabbit polyclonal to NUAK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human NUAK2 aa 450 to the C-terminus.
    Database link: Q9H093

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: NUAK2 over-expression HEK-293T whole cell lysate; ICC: U-251 MG cells; IHC-P: Human cervix, duodenum, testis and cerebellum tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Other

Images

  • Western blot - Anti-NUAK2 antibody (ab224079)
    Western blot - Anti-NUAK2 antibody (ab224079)
    All lanes : ab224079 at 0.4 µg/ml

    Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : NUAK2 over-expression HEK-293T whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

    Predicted band size: 69 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUAK2 antibody (ab224079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUAK2 antibody (ab224079)

    Immunohistochemical analysis of human cervix tissue labeling NUAK2 in the nucleus of squamous epithelial cells with ab224079 at 1/50 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUAK2 antibody (ab224079)
    Immunocytochemistry/ Immunofluorescence - Anti-NUAK2 antibody (ab224079)

    Immunofluorescent analysis of U-251 MG (human brain glioma cell line) cells labeling NUAK2 (green) in the nucleoplasm, nucleoli fibrillar center and cytosol with ab224079 at 2 µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUAK2 antibody (ab224079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUAK2 antibody (ab224079)

    Immunohistochemical analysis of human duodenum tissue labeling NUAK2 in the glandular cells with ab224079 at 1/50 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUAK2 antibody (ab224079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUAK2 antibody (ab224079)

    Immunohistochemical analysis of human testis tissue labeling NUAK2 in the nucleus of cells in seminiferous ducts with ab224079 at 1/50 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUAK2 antibody (ab224079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUAK2 antibody (ab224079)

    Immunohistochemical analysis of human cerebellum tissue labeling NUAK2 in neuronal porcesses with ab224079 at 1/50 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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