Chicken Alpha 1 Acid Glycoprotein / AGP ELISA Kit (ab157690)
Key features and details
- Sensitivity: 1.619 ng/ml
- Range: 6.25 ng/ml - 200 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Chicken
Overview
-
Product name
Chicken Alpha 1 Acid Glycoprotein / AGP ELISA Kit
See all Alpha 1 Acid Glycoprotein/AGP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Other biological fluids -
Assay type
Sandwich (quantitative) -
Sensitivity
= 1.619 ng/ml -
Range
6.25 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Chicken -
Product overview
Abcam’s Alpha 1 Acid Glycoprotein / AGP Chicken ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Alpha 1 Acid Glycoprotein / AGP in biological samples of chicken.
In this assay the Alpha 1 Acid Glycoprotein / AGP present in samples reacts with the anti-Alpha 1 Acid Glycoprotein / AGP antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Alpha 1 Acid Glycoprotein / AGP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1 Acid Glycoprotein / AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1 Acid Glycoprotein / AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1 Acid Glycoprotein / AGP in the test sample. The quantity of Alpha 1 Acid Glycoprotein / AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-conjugated anti-chicken Alpha 1 Acid Glycoprotein / AGP antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chicken Alpha 1 Acid Glycoprotein / AGP Calibrator (Lyophilized) 1 vial Chicken Alpha 1 Acid Glycoprotein / AGP ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Stop Solution 1 x 12ml -
Research areas
-
Function
Appears to function in modulating the activity of the immune system during the acute-phase reaction. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- A1AG1_HUMAN
- AGP
- AGP 1
see all
Images
-
Standard Curve
Representative standard curve using ab157690.