CETP Activity Assay Kit II (Fluorometric) (ab196995)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Plasma, Purified protein, Serum
Overview
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Product name
CETP Activity Assay Kit II (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Purified protein -
Assay type
Enzyme activity -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
CETP Activity Assay Kit II (Fluorometric) (ab196995) uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of Cholesteryl Ester Transfer Protein (CETP). CETP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Ex/Em = 480/511 nm). The fluorometric intensity is directly proportional to the amount of phospholipid transferred. The kit contains rabbit serum and torcetrapib (CETP inhibitor) as positive and negative control, respectively, for assay validation.
This assay can measure PLTP activity in plasma and serum but can also be used for testing activity of recombinant CETP protein.
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Notes
Cholesteryl Ester Transfer Protein (CETP) is a member of the lipid transfer/lipopolysaccharide binding protein gene family. CETP is plasma protein that transfers a cholesteryl ester from HDL to LDL or VLDL in exchange for a triglyceride. HDL plays an important role in lipid metabolism and cardiovascular health. HDL transports cholesterol to the liver for excretion or to steroidogenic tissues for steroid synthesis. HDL also plays an important role in the reverse cholesterol transport pathway, removing cholesterol from lipid-filled macrophages, protecting against atherosclerosis. Because of this function, CETP is viewed as a target to increase HDL, with CETP inhibition an active area of research and several CETP inhibitors at various stages of drug development.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests CETP Acceptor Molecule 1 x 0.5ml CETP Assay Buffer 1 x 20ml CETP Inhibitor 1 x 10µl CETP Positive Control 1 x 0.1ml Donor Molecule (8 nmol/mL) 1 x 0.5ml -
Research areas
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Relevance
Cholesteryl ester transfer protein is a member of the lipid transfer/lipopolysaccharide binding protein gene family. Cholesteryl ester transfer protein transfers neutral lipids from high-density lipoprotein (HDL) to very low-density lipoprotein (VLDL) and is present in normal human plasma and serum. -
Cellular localization
Secreted, extracellular space. -
Alternative names
- CETP
- HDLCQ10
- Lipid transfer protein
- Lipid transfer protein I
Images
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Functional Studies - CETP Activity Assay Kit II (Fluorometric) (ab196995)
Typical Donor molecule Standard Curve obtained following assay protocol.
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Functional Studies - CETP Activity Assay Kit II (Fluorometric) (ab196995)Measurement of CETP activity on 1 µL rabbit serum (green), 1 µL and human plasma (yellow) 4 µL. Background control with no serum is shown (blue).
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Functional Studies - CETP Activity Assay Kit II (Fluorometric) (ab196995)Inhibition curve of CETP activity from rabbit serum by Torcetrapib (CETP inhibitor). The assay was run for 1 hour and the IC50 was determined to be 3.56 nM.
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Functional Studies - CETP Activity Assay Kit II (Fluorometric) (ab196995)Inhibition of CETP activity from human plasma using 80 nM Torcetrapib; assay was run for 2 hours following protocol instructions.
