Cellular Calcineurin Phosphatase Activity Assay Kit (Colorimetric) (ab139464)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture extracts, Tissue Extracts
Overview
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Product name
Cellular Calcineurin Phosphatase Activity Assay Kit (Colorimetric)
See all Calcineurin A kits -
Detection method
Colorimetric -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Enzyme activity -
Product overview
Calcineurin Activity Assay Kit (Colorimetric) ab139464 is designed for measuring cellular calcineurin (CaN, PP2B) phosphatase activity. It employs a convenient 96-well microtiter-plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts.
The detection of free-phosphate released in the calcineurin assay protocol is based on the classic Malachite green assay and offers the following advantages: non-radioactive; convenient 1-step detection; excellent sensitivity.
This kit includes human recombinant calcineurin as a positive control. The RII phosphopeptide substrate (Calcineurin Substrate), supplied with this calcineurin assay, is the most efficient and outstanding peptide substrate known for calcineurin.
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Notes
Previously called Cellular Calcineurin Phosphatase Activity Assay Kit (Colorimetric).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 2X EGTA Buffer 1 x 1ml 96-well Clear Microplate (1/2 Volume) 2 units Active Calcineurin Enzyme 1 x 10µl Calcineurin Assay Buffer 1 x 20ml Calcineurin Substrate 1 x 1.5mg Calmodulin 1 x 100µl Desalting Column 1 unit Desalting Resin 1 x 1g Green Assay Reagent 1 x 20ml Lysis Buffer 1 x 40ml Okadaic Acid 1 x 325µl Phosphate Standard 1 x 0.5ml Protease Inhibitor Cocktail 1 x 2 tablets -
Research areas
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Function
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-2B subfamily. -
Cellular localization
Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. - Information by UniProt
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Alternative names
- Alpha isoform formerly PPP2B
- Calcineurin A alpha
- Calcineurin A1
see all
Images
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Green assay phosphate standard curve
Example of standard curve obtained with ab139464.
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Cellular Calcineurin Assay using Mouse brainPhosphatase activity from a freshly prepared Mouse brain extract. Prior to gel filtration the extract was diluted 1/1 in lysis buffer containing protease inhibitors. After gel filtration and prior to the phosphatase assay, the extract was diluted 1/25 in lysis buffer. The reaction was incubated for 30 min at 30°C.
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Calcineurin positive controlPurified calcineurin positive control. The Calcineurin was incubated 1 hr at 30°C under various buffer conditions. The results demonstrate that Calcineurin activity is inhibited by the EGTA buffer, but not inhibited by 100 nM or 500 nM concentrations of okadaic acid.
