CAPZB overexpression 293T lysate (whole cell) (ab94201)
Overview
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Product name
CAPZB overexpression 293T lysate (whole cell) -
General notes
ab94201 is a 293T cell transfected lysate in which Human CAPZB has been transiently over-expressed using a pCMV-CAPZB plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Similarity: Belongs to the F-actin-capping protein beta subunit family.
Images
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SDS-PAGE - CAPZB overexpression 293T lysate (whole cell) (ab94201)ab94201 at 15µg/lane on an SDS-PAGE gel.
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Western blot - CAPZB overexpression 293T lysate (whole cell) (ab94201)All lanes : Anti-CAPZB antibody (ab54930) at 1/500 dilution
Lane 1 :CAPZB overexpression 293T lysate (whole cell) (ab94201)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution