Canine Resistin ELISA Kit (ab273177)
Key features and details
- Sensitivity: 16 pg/ml
- Range: 16.38 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine Resistin ELISA Kit
See all Resistin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16 pg/ml -
Range
16.38 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 138.2 129% - 149% Plasma 118.4 85% - 150% Cell culture media 87.49 82% - 95% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine Resistin ELISA Kit (ab273177) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine Resistin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine Resistin coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine Resistin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine Resistin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine Resistin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Anti-Canine Resistin coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Canine Resistin Antibody 2 vials Canine Resistin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
Tissue specificity
Expressed only in fatty tissues. -
Sequence similarities
Belongs to the resistin/FIZZ family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
see all
Images
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Example Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
