Canine IgG ELISA Kit (ab193768)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 100 pg/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine IgG ELISA Kit
See all IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 3 2.7% Inter-assay Sample n Mean SD CV% Serum 3 3.2% -
Sample type
Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
100 pg/ml -
Range
0.31 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture media 101 98% - 106% Fetal Bovine Serum 117 112% - 128% 1X Wash Buffer 112 109% - 118% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine IgG ELISA Kit (ab193768) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgG protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Canine IgG with 100 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
There are six classes of immunoglobulins in canine: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin in serum and is equally distributed in blood and tissue. In canine, the IgG class is further divided into four subclasses: IgG1, IgG2, IgG3, and IgG4. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y ike structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane.
IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens and also plays an important role in antibody dependent cell-mediated cytotoxicity. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Canine IgG Capture Antibody 1 x 600µl 10X Canine IgG Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CP 1 x 6ml Canine IgG Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
Images
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Other - Canine IgG ELISA Kit (ab193768)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of canine IgG standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of canine serum IgG level in Sample Diluent NS.Background-subtracted data values (mean +/- SD, n = 3) are graphed.
