Canine IgG ELISA Kit (ab157701)
Key features and details
- Sensitivity: 7.148 ng/ml
- Range: 25 ng/ml - 800 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine IgG ELISA Kit
See all IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Other biological fluids -
Assay type
Sandwich (quantitative) -
Sensitivity
7.148 ng/ml -
Range
25 ng/ml - 800 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Abcam’s Canine IgG ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG in biological samples of dog.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-conjugated anti-dog IgG antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Dog IgG Calibrator (Lyophilized) 1 vial Dog IgG ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
Images
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Standard CurveRepresentative standard curve using ab157701 IgG Dog ELISA kit
