Anti-Tex19.1 antibody (ab129726)
Key features and details
- Rabbit polyclonal to Tex19.1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Tex19.1 antibody -
Description
Rabbit polyclonal to Tex19.1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Tex19.1 aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: Q99MV2 -
Positive control
- This antibody gave a positive signal in both Mouse Testis and Placenta tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Target
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Relevance
The Human testis expressed gene 19 (Tex19) is duplicated in mouse and rat to give two genes Tex19.1 and Tex19.2. Tex19.1 expression is limited to pluripotent cells and germ cells, and is downregulated after differentiation. The Tex19.1 (-/-) knockout male mice have impaired spermatogenesis. -
Database links
- Entrez Gene: 73679 Mouse
- SwissProt: Q99MV2 Mouse
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Alternative names
- Testis expressed protein 19A antibody
- Tex 19.1 antibody
- Tex 19a antibody
- Tex19a antibody
Images
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Western blot - Anti-Tex19.1 antibody (ab129726)All lanes : Anti-Tex19.1 antibody (ab129726) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Mouse Placenta Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 45 kDa (possible non-specific binding)
Exposure time: 8 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129726 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab129726 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Tex19.1 antibody (ab129726)All lanes : Anti-Tex19.1 antibody (ab129726) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Mouse Placenta Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 45 kDa (possible non-specific binding)
Exposure time: 8 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129726 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
