Canine HGF ELISA Kit (ab273174)
Key features and details
- Sensitivity: 0.24 ng/ml
- Range: 0.246 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine HGF ELISA Kit
See all HGF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.24 ng/ml -
Range
0.246 ng/ml - 60 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94.38 79% - 116% Plasma 100.6 86% - 123% Cell culture media 126.5 110% - 145% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine HGF ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine HGF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Canine HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Canine HGF coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine HGF Antibody 2 vials Canine HGF Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. -
Involvement in disease
Deafness, autosomal recessive, 39 -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
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Alternative names
- DFNB39
- F TCF
- Fibroblast derived tumor cytotoxic factor
see all -
Database links
- Entrez Gene: 403441 Dog
- SwissProt: Q867B7 Dog
Images
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Canine HGF ELISA Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Canine HGF ELISA kit (ab273174) Standard curve.