Canine EPO ELISA kit (ab277400)
Key features and details
- Sensitivity: 0.085 ng/ml
- Range: 0.082 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine EPO ELISA kit
See all EPO kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% General Inter-assay Sample n Mean SD CV% General -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.085 ng/ml -
Range
0.082 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 121.2 109% - 137% Serum 122.3 101% - 143% Plasma 111.2 87% - 147% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine EPO ELISA Kit (ab277400) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine EPO in serum (Canine EPO concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Canine EPO coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine EPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine EPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine EPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Canine EPO Antibody 2 vials Anti-Canine EPO coated Microplate 1 unit Canine EPO standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. -
Cellular localization
Secreted -
Alternative names
- EP
- EPO
- EPO alpha
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Images
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Canine EPO ELISA kit (ab277400) Standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
